Coding

Part:BBa_K2960009:Design

Designed by: Ann Metzloff   Group: iGEM19_Cornell   (2019-10-16)


The mlrD transporter protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 126
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 310
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mlrD gene sequence has been codon-optimized for expression in E. coli.

The construct was designed to be RFC[10] compatible.

Source

Sphingopyxis sp. genomic sequence. European Nucleotide Archive. (2019 April 5). Received from https://www.ebi.ac.uk/ena/data/view/QCT05772

References

Zhang, J., Lu, Q., Ding, Q., Yin, L., & Pu, Y. (2017). A Novel and Native Microcystin-Degrading Bacterium of Sphingopyxis sp. Isolated from Lake Taihu. International journal of environmental research and public health, 14(10), 1187. doi:10.3390/ijerph14101187